Glass slides to DNA microarrays

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Glass slides to DNA microarrays

on genomics, became aware of the potential commercial, technical, and social importance of the human genome project during the late 1990s. The human genome project was formally initiated in 19901 and was expected to last 15 years. It had the major goals of identifying all the genes in human DNA, determining the sequences of those genes, and storing the information in public databases. However, ...

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Dendrimeric coating of glass slides for sensitive DNA microarrays analysis.

Successful use and reliability of microarray technology is highly dependent on several factors, including surface chemistry parameters and accessibility of cDNA targets to the DNA probes fixed onto the surface. Here, we show that functionalisation of glass slides with homemade dendrimers allow production of more sensitive and reliable DNA microarrays. The dendrimers are nanometric structures of...

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development of polymer-coated glass slides as optical oligonucleotide microarrays

background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...

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An Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays

Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...

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Development of Polymer-Coated Glass Slides as Optical Oligonucleotide Microarrays

BACKGROUND The microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. METHODS In this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. For fabrication of these substrates, a thin film of ox...

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ژورنال

عنوان ژورنال: Materials Today

سال: 2004

ISSN: 1369-7021

DOI: 10.1016/s1369-7021(04)00122-1